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    <title>UTas ePrints - Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea</title>
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    <meta content="Reid, James B." name="eprints.creators_name" />
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<meta content="O'Neill, Damian P." name="eprints.creators_name" />
<meta content="Kerckhoffs, L. Huub J." name="eprints.creators_name" />
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<meta content="Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation." name="eprints.abstract" />
<meta content="2002" name="eprints.date" />
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<meta content="Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman
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Ait-Ali T, Frances S, Weller JL, Reid JB, Kendrick RE,
Kamiya Y (1999) Regulation of gibberellin 20-oxidase
and gibberellin 3{3-hydroxylase transcript accumulation
during de-etiolation of pea seedlings. Plant Physiol 121:
783-791
Ait-Ali T, Swain SM, Reid JB, Sun T, Kamiya Y (1997)The
LS locus of pea encodes the gibberellin biosynthesis enzyme
ent-kaurene synthase A. Plant J 11: 443-454
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pea. J Plant Growth Regul 20: 87-94
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Phillips AL, Ward DA, Gaskin P, Hedden P (1997)
Isolation and transcript analysis of gibberellin 20-oxidase
genes in pea and bean in relation to fruit development.
Plant Mol BioI 33: 1073-1084
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GA 20-oxidase and GA 3 {3-hydroxylase in etiolated pea
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Y (1998) Phytochrome regulates gibberellin biosynthesis
during germination of photoblastic lettuce seeds.
Plant Physiol 118: 1517-1523
Weller JL, Beauchamp N, Kerckhoffs LHJ, Platten JD, Reid JB (2001) Interaction of phytochromes A and B in the control of de-etiolation and flowering in pea. Plant J
26: 1-14
Weller JL, Murfet IC, Reid JB (1997) Pea mutants with
reduced sensitivity to far-red light define an important
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(1995) New lu mutants of pea are deficient in phytochrome
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(1998) The role of gibberellin, abscisic acid, and sucrose
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" name="eprints.referencetext" />
<meta content="Reid, James B. and Botwright, Natasha A. and Smith, Jennifer J. and O'Neill, Damian P. and Kerckhoffs, L. Huub J. (2002) Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea. Plant Physiology, 128 (2). pp. 734-741. ISSN 0032-0889" name="eprints.citation" />
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<meta content="Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation." name="DC.description" />
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    <h1 class="ep_tm_pagetitle">Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Reid, James B.</span> and <span class="person_name">Botwright, Natasha A.</span> and <span class="person_name">Smith, Jennifer J.</span> and <span class="person_name">O'Neill, Damian P.</span> and <span class="person_name">Kerckhoffs, L. Huub J.</span> (2002) <xhtml:em>Control of Gibberellin Levels and Gene Expression during De-Etiolation in Pea.</xhtml:em> Plant Physiology, 128 (2). pp. 734-741. ISSN 0032-0889</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/2369/1/Control_of_Gibberellin.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />2840Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="3053" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://proquest.umi.com/pqdweb?did=109212662&amp;sid=1&amp;Fmt=4&amp;clientId=20931&amp;RQT=309&amp;VName=PQD">http://proquest.umi.com/pqdweb?did=109212662&amp;sid=1&amp;Fmt=4&amp;clientId=20931&amp;RQT=309&amp;VName=PQD</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Gibberellin Al (GAl) levels drop significantly in wild-type pea (Pisum sativum) plants within 4 h of exposure to red, blue, or far-red light. This response is controlled by phytochrome A (phyA) (and not phyB) and a blue light receptor. GAs levels are increased in response to 4 h of red light, whereas the levels of GA19, GA2o, and GA29 do not vary substantially. Red light appears to control GAl levels by down-regulating the expression of Mendel's LE (PsGA30xl) gene that controls the conversion of GA20 to GAl' and by up-regulating PsGA2ox2, which codes for a GA 2-oxidase that converts GAl to GAs. This occurs within 0.5 to 1 h of exposure to red light. Similar responses occur in blue light. The major GA 20-oxidase gene expressed in shoots, PsGA20oxl, does not show substantial light regulation, but does show up-regulation after 4 h of red light, probably as a result of feedback regulation. Expression of PsGA30xl shows a similar feedback response, whereas PsGA2ox2 shows a feed-forward response. These results add to our understanding of how light reduces shoot elongation during de-etiolation.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/320000.html">320000 Medical and Health Sciences</a></td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2369</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Scholarly Publications Librarian</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">31 Oct 2007 08:51</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">09 Jan 2008 02:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2369;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2369">item control page</a></p>
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